1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.

2. Technical specifications
Sensitivity : 0.5 ppb
Detection limit:
Honey 1.5 ppb
Meat, liver, shrimp, fish 0.5 ppb
Recovery rate:
Honey 70 ±10%
Meat, liver, shrimp, fish 80 ±10%
Cross-reaction rate:
Florfenicol 100%
Florfenicol Amine 11%
Thiamphenicol < 0.1%
Chloramphenicol < 0.1%

3. Components
1ï¼Â‰ Micro-well strips: 12 strips with 8 removable wells each
2ï¼Â‰ 6?standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
3ï¼Â‰ Enzyme conjugate (12 mL) red cap
4ï¼Â‰ Antibody working solution (7 mL) blue cap
5ï¼Â‰ Substrate A solution (7 mL) white cap
6ï¼Â‰ Substrate B solution (7 mL) black cap
7ï¼Â‰ Stop solution (7 mL) yellow cap
8ï¼Â‰ 20?concentrated washing buffer (40 mL) white cap
9ï¼Â‰ 2?concentrated redissolving solution (50 mL) transparent cap